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Why Peptide Stability Matters Peptide stability refers to how well a peptide maintains its expected analytical profile over time

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Next, the gels were equilibrated in transfer buffer (25 mM Tris base, 192 mM glycine, and 10% methanol) for 30 min at 4C, after which the proteins were transferred to polyvinylidene fluoride membranes (Bio-Rad Laboratories) at a constant current of 300 mA for 3 h at 4C

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